Click image to see more details
Product Info Summary
| SKU: | EKC1292 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
Customers Who Bought This Also Bought
Product info
Product Name
IKK-alpha Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1292
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The IKK-alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
IKK-alpha Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1292)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1292 is reactive to CHUK in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1292 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-CHUK Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from 293 cells
Protein Target Info & Infographic
Gene/Protein Information For CHUK (Source: Uniprot.Org, NCBI)
Gene Name
CHUK
Full Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Weight
84.64kDa
Superfamily
protein kinase superfamily
Alternative Names
CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK alpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16 CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CHUK, check out the CHUK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CHUK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IKK-alpha Colorimetric Cell-Based ELISA Kit (EKC1292)
Hello CJ!
No publications found for EKC1292
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used IKK-alpha Colorimetric Cell-Based ELISA Kit?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review IKK-alpha Colorimetric Cell-Based ELISA Kit
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for IKK-alpha Colorimetric Cell-Based ELISA Kit
Question
Do the EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit recognize an extracellular epitope or do you have to follow an intracellular protocol for flow?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
For EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit and most of our antibodies we are not able to provide specific epitope information but it seems most targets are intracellular. Therefore, not on the membrane and cells need fixation prior to staining intracellular target. Flow cytometry protocol is recommended.
Boster Scientific Support
Answered: 2019-12-13
Question
I have a question regarding your EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit. I want to use antibody for flow cytometric analysis of phosphorylated IKK-alpha, do this EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit detect phosphorylated IKK-alpha or only IKK-alpha? Since I want to study the TLR signaling pathway and I need to look at activated (phosphorylated) IIKK alpha enzyme. Can you guide me regarding this...so I can order an antibody accordingly?
Verified Customer
Verified customer
Asked: 2013-02-20
Answer
We can confirmed that EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit has not been specifically raised against a phosphorylated residue of the protein, it is therefore likely to detect both phosphorylated and non-phosphorylated forms of the protein. We recommend to review the datasheets for further details and to select the tested antibody in the species you will be using. In regards to flow cytometry, unfortunately EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit has not been tested, so we cannot guarantee.
Boster Scientific Support
Answered: 2013-02-20
Question
Hello; What is the concentration of EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit?
Verified Customer
Verified customer
Asked: 2013-01-10
Answer
The concentration for EKC1292 IKK-alpha Colorimetric Cell-Based ELISA Kit is 0.25mg/ml.
Boster Scientific Support
Answered: 2013-01-10
